Our new article has been accepted in Bioinformatics:
Schwarz M, Welzel M, Kabdullayeva T, Becker A, Freisleben B, Heider D: MESA: automated assessment of synthetic DNA fragments and simulation of DNA synthesis, storage, sequencing, and PCR errors. Bioinformatics 2020, in press. (Link)
The development of de novo DNA synthesis, PCR, DNA sequencing, and molecular cloning gave researchers unprecedented control over DNA and DNA-mediated processes. To reduce the error probabilities of these techniques, DNA composition has to adhere to method-dependent restrictions. To comply with such restrictions, a synthetic DNA fragment is often adjusted manually or by using custom-made scripts. In this paper, we present MESA (Mosla Error Simulator), a web application for the assessment of DNA fragments based on limitations of DNA synthesis, amplification, cloning, sequencing methods, and biological restrictions of host organisms. Furthermore, MESA can be used to simulate errors during synthesis, PCR, storage, and sequencing processes.
MESA is available at mesa.mosla.de, with the source code available at github.com/umr-ds/mesa_dna_sim.